Screening of some nigerian plants for pyrrolizidine alkaloids

Authors

  • T. A. Dare Department of Pharmacology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • N. Nwude Department of Pharmacology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • P. I. Rekwot Animal Production Research Programme, National Animal Production Research Institute, Ahmadu Bello University, Zaria, Nigeria
  • M. Mamman Department of Pharmacology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria
  • B. O. Omontese Department of Theriogenology and Production, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

Keywords:

Pyrrolizidine alkaloids; Ehrlich’s reagent; Plants; Nigeria

Abstract

Pyrrolizidine alkaloids (PAs) are an important class of plant toxins whose hepato- and pneumo-toxicity have been extensively studied.  Twenty three field plant materials (leaves, flowers, pulp, seeds) were collected, identified and screened for unsaturated PAs. A field test method with Ehrlich reagent was used. The absorbance of Ehrlich colour of the PAs and N-oxides were obtained at 565nm. Positive reactions were obtained from Borreria verticillata; Guiera senegalensis; Tamarindus indicaCrotolaria doniana; C. gorensis; C. glaucoides; C. mucronata; C. naragutensis; C. lachnosema and C. retusa. The occurrence of PAs are reported for the first time in C. doniana and C. glaucoides.  B. verticillata,  G. senegalensis and T. indica gave positive Ehrlich reactions which could be due to PAs or other groups giving positive Ehrlich reaction. A number of medicinal and food plants gave negative Ehrlich reaction. Further work is required to confirm and identify the alkaloids in the plants that gave positive reactions.

References

Adaudi, A.O., 1975. Dichepalatum barteri poisoning in Goats. Trop. Anim. Health Prod. 7, 56-57.

Abatan, M.O., Arowolo, R.O.A., Olorunsogo, O., 1996. Pathological effects of Lantana camara and Dichapelatum

madagascasiene in Goat. Trop. Vet. 15, 49-54.

ANZFA. 2001. Australia New Zealand Food Authority. Pyrrolizidine alkaloids in food: A toxizoological review and

risk assessment. Technical Report No. 2.

Crews, C., Stanin, J.R., Clarke, P.A., 1997. Determination of Pyrrolizidine alkaloids in honey from selected sites by

solid phase extraction and HPLC- MS. Food Additives and Contaminants. 14, 419-428.

Culvenor, C.C.J., Edgar, J.A., Smith, L.W., 1981. Pyrrolizidine alkaloids, in honey from Echium plantagineum L. J. of

Agric. and Food Chem. 29, 958-960.

Dalziel, J.H., 1937. The useful Plants of West Tropical Africa, Crown agents for Overseas Governments and

Administration, London, pp. 79-80.

Deinzer, M.L., Thomason, P.A., Burgett, D.M., Isaacson, D.L., 1977. Pyrrolizidine alkaloids: Their occurrence in

honey from Tansy Ragwort (S. jacobea L) Science, 195, 497-499.

Dharmananda, S., 2002. Safety issues affecting herbs: Pyrrolizidine alkaloids.

http://www.itmonline.org/arts/pas.htm.

Dickinson, J.O., Cooke, M.P., King, R.R., Mohamed, P.A., 1976. Milk Transfer of Pyrrolizidine alkaloids in cattle. J. of

Amer. Vet. Med. Assoc. 169, 1192-1196.

Edgar, J.A., Smith, W.S., 2002. Transfer of Pyrrolizidine alkaloids into eggs: Food safety implications. In: Natural

and Selected Synthetic Toxins: Biological Implications ACS Symposium Series 745. American Chemical Safety,

Washington, DC. Pp, 118-128.

Fu, P.P., Yang, Y.C., Xia, Q., Chou, M.C., Cui, Y.Y., Lin, G., 2002. Pyrrolizidine alkaloids tumorgenic components in

Chinese herbal medicines and dietary supplements. J. of Food and Drug Analysis, 10 (4), 198-211.

Hooper, P.T., 1978. Pyrrolozidine alkaloid poisoning. Pathology with particular reference to differences in animal

and plant species. In: Effects of Poisonous Plants on Livestock. R.F. Keeler, K.R. VanKampen and I. F. James

(Eds) Pp 161-176. Academic Press, New York.

Kellerman, T.S., Fourie, N., Naude, T.W., 1996. Plant Poisonings and Mycotoxicoses of Livestock in Southern Africa.

Oxford University Press, Cape Town.

Mattocks, A.R., 1986. Chemistry and Toxicology of Pyrrolizidine Alkoloids, Academic Press Orlando, FL.

Mattocks, A.R., Nwude, N., 1988. Pyrrolizidine alkaloids from Crotolaria lachnosema and C. naragutensis.

Phytochemistry, 27(10), 3289-3291.

Mattocks, A.R., Culvenor, C.C.J., Tandon, H.J., Prost, A., 1988. Environmental health criteria for Pyrrolizidine

alkaloids. World Health Organization Geneva. http://www.unchem.org/documents/ehc/ehc/080htm.

Molyneux, R.J., James, L.F., 1990. Pyrrolizidine alkaloids in milk: Thresholds of intoxication. Vet. and Human

Toxicology J., 32, 94-103.

Nwude, N., 1986. Poisonous Plants in Nigeria. Private Edition pp. 189.

Ober, D., Hartmann, N., 1999. Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine

alkaloid biosynthesis, evolved from deoxyhypusine synthase. Proc. in National Academic Sci. 96 (26), 14777-

Onyeyili, P.A., Chibuzo, G.A., Egwu, G.O., 1994. Accidental poisoning of sheep in an arid zone of Nigeria. World

Animal Review. Pp 78.

Otesile, E.B., Akpkodje, J.U., 1986. Clinical observations on Leucaena leucocephala (L.A.M) DE WIT Toxicity in

Nigerian White Fulani Cattle. Trop. Vet. Med. 4, 75-77.

Radominska –Padya, A., 2010. “Invited speakers” Drug metabolism Review, 42 (1), 1.

RIMS, 1992. Nigeria Livestock Resources. Four volume report submitted to the Federal Government of Nigeria by

Resources Inventory Management Limited.

Roulet, M., Laurini, R., Rivier, L., Calame, A., 1988. Hepatic Veno-Occlusive disease in newborn infant of a woman

drinking herbal tea. J. of Pediatrics, 112, 433-436.

Smith, L.W., Culvenor, C.C., 1981. Plant Sources of Hepatotoxic Pyrrolozidine alkaloids. J. of Natural Products, 44,

-152.

Steenkamp, V., Stewart, M.J., Zuckerman, M. 2000. Clinical and analytical aspects of Pyrrolizidine alkaloids

Poisoning caused by South Africa traditional medicine. Therapetic Drug monitoring, 22, 302-306.

Stegelmeier, B.L., Edgar, J.A., Colegate, S.M., Gardener, D.R., Schoch, T.K., Conlombe, R.A., Molyneux, R.J. 1999.

Pyrrolizidine alkaloid plants, metabolism and toxicity. J. of Natural Toxins 8 (1), 95-116.

Stillman, A.F., Huxtable, R., Consroe, P., Kehnen, P., Smith, S. 1977. Hepatic veno-occlusive disease due to

pyrrolizidine (senecio) poisoning in Arizona. Gastroenterology, 73, 349-352.

Sperl, W., Stuppner, H., Cassner, I., Judmaier, W., Dietze, O., Vogel, W. 1995. Reversible hepatic veno-occlusive

disease in an infant after consumption of pyrrolizidine containing herbal tea. European J. of Pediatrics, 154,

-116.

Tandon, H.D., Tandon, B.N., Mattocks, A.R. 1978. An epidemic of Veno-occlusive disease of the liver in Afghanistan.

Pathologic features. Amer. J. of Gastroenterology, 70, 607-613.

Published

2013-06-29

How to Cite

A. Dare, T., Nwude, N. ., I. Rekwot, P., Mamman, M. ., & O. Omontese, B. . (2013). Screening of some nigerian plants for pyrrolizidine alkaloids. Scientific Journal of Animal Science, 2(6), 138-143. Retrieved from https://www.sjournals.com/index.php/sjas/article/view/1076

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