Toxicity and analgesic studies of leaf methanolic extract of Maytenus Senegalensis (LAM.) Exell (Celastraceae)
Keywords:oxicity, analgesic, methanolic extract, Maytenus senegalensis, Celastraceae, acetic acid-induced writhing, formalin induced pain
The leaf methanolic extract of Maytenus senegalensis (Lam.) Exell was investigated for toxicity and analgesic effects. Acute toxicity was investigated via intraperitoneal route using mice and rats, analgesic activity was investigated using two models; acetic acid-induced writhing in mice and formalin induced pain in rats. The extract was determined to have LD (50) of 1264.91 mg/kg in both mice and rats. It significantly (0.001) inhibited acetic acid-induced writhes in mice at all doses administered (75, 150 and 300 mg/kg) in a dose dependent manner and significantly (0.01) inhibited formalin induced pain in rats, in a non-dose dependent manner. The inhibitory effect observed was higher in the extract compared to the standard used (piroxicam). The results obtained suggested the extract to be relatively toxic in both mice and rats with a dose dependent anti-nociceptive activity.
Bentley, G.A., Newton, S.H., Starr, J., 1983. Studies on the anti-nociceptive action of agonist drugs and their
interaction with opiod mechanisms. British J. Pharmacol., 32, 295-310.
Burkill, H.M., 1985. The useful plants of west tropical Africa. Vol. 1. Royal Botanical Gardens Kew, United kindom.
Chant, S.R., 1993. Celastraceae. In: Heywood, V. H. (ed.) Flowering plants of the world. New York: Oxford
Dubuisson, D., Dennis, S.R., 1977. The formalin test: A quantitative study of the analgesic effects of
morphine, meperidine, and brain stem stimulation in rats and cats., Pain, 4, 161-174.
Gaertner, M., Muller, L., Roos, J. F., Cani, G., Santos, A.R., Niero, R., Calixto, J.B., Yunes, R.A., Delle Monache,
F., Cechinel Filho, V., 1999. Analgesic triterpenes from Sebastiania schottiana roots. Phytomed., 6, 41- 44.
Ibrahim, G., Abdurahman, E.M., Ibrahim, H., Ibrahim, N.D.G., Magaji, M.G., 2011. Toxicity And Analgesic Effects Of
Vernonia Amygdalina Del. (Asteraceae) Leaf Extract On Mice. Inter. J. Adv. Pharmaceut. Biolog. Sci., 1(1), pp.
Koster, R., Anderson, M., De Beer, E.J., 1959. Acetic acid for analgesic screening. Federat. proceed., 18, 412.
Lorke, D., 1983. A new approach to acute toxicity testing. Arch. Toxicol., 54, 275-287.
Martin, A.E., 2002. Oxford Medical Dictionar,. 3rd ed. Oxford Univ. Press., p. 16.
Matsumma, F., 1975. Toxicology of insecticides plenum press. York and London., pp 24-2.
Tjölsen, A., Berge, O., Hunskaar, S., Rosland, J.H., Hole, K., 1992. The formalin test: an evaluation of the method.
Pain., 52, 5-17.
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