In vivo phytotherapy of snail by plant derived active components in control of fascioliasis

Authors

  • Kumari Sunita Malacology laboratory, Department of Zoology, DDU Gorakhpur University Gorakhpur 273009 (U.P), India
  • Pradeep Kumar Malacology laboratory, Department of Zoology, DDU Gorakhpur University Gorakhpur 273009 (U.P), India
  • D. K. Singh Malacology laboratory, Department of Zoology, DDU Gorakhpur University Gorakhpur 273009 (U.P), India

Keywords:

Lymnaea acuminate, Redia, Cercaria, Plant derived larvicidal, Phytotherapy

Abstract

Fascioliasis is worldwide zoonotic disease, which caused great economic loss to human being. In vivo killing of Fasciola larva (redia and cercaria) is one of the effective methods to control of fascioliasis. In vivo toxicity of different binary combinations (1:1ratio) of plant derived active components such as citral, ferulic acid, umbelliferone; azadirachtin and allicin were tested against Fasciola larva  (redia and cercaria) in snail Lymnaea acuminata. Mortality of larvae were observed at 2h, 4h, 6h and 8h of treatment. In vivo exposure of azadirachtin + allicin (1:1 ratio) was highly toxic against redia and cercaria (8h LC50 0.007and 0.006 mg/L). Combinations of citral + umbelliferone, cirtal + ferulic acid were less toxicity against redia and cercaria larva than azadirachtin + allicin. In vivo use of these larvicidal active components in the binary combinations at sublethal does will be more helpful in controlling the redia/cercaria than their individual components.

References

Adewunmi, C.O., Oguntimein, B.O., Furu, P., 1990. Molluscicidal and antischistosomal activities of Zingiber

officinale. Planta Medica, 56(4), 374- 376.

Agarwal, R.A., Singh, D.K., 1988. Harmful Gastropods and their control. Acta Hydrochim Hydrobiol, 16, 113-138.

Assis, L.M., Bevilequa, C.M.L., Morais, S.M., Vieira, L.S., Costa, C.T.C., Souza, J.A.L., 2003. Ovicidal and larvicidal

activity in vitro of Spieled anthelia Linn. Extract on Haemonchus contorlus. Veterinary Parasitology, 117, 43-

Datta, A., Sukul, N.C., 1987. Antifilarial effect of Zingiber officinale on Dirofilaria immitis. Journal of Helminthology,

, 255-258.

Eigner, D., Scholz, D., 1999. Ferula asafoetida and Curcuma longa in traditional treatment and diet in Nepal.

Journal of Ethnopharmacology, 67, 1-6.

Fatehi, M., Farifteh, F., Hassanabad, Z.F., 2004. Antispasmodic and hypotensive effect Ferula asafoetida gum

extract. Journal of Ethnopharmacology, 91, 321-324.

Goto, C., Kasuya, S., Koga, K., Ohtomo, H., Kaget, N., 1990. Lethal efficacy of extract from Zingiber officinale

(traditional Chinese medicine) or [6]-shogaol and [6]-gingerol in Anisakis larvae in vitro. Parasitology

Research, 76, 653- 656.

Hoppe, H.A., 1975. Drogenkunde, vol. I .Angiosperms. 8thEd, Walter De Gruyter, Berlin.

Jaiswal, P., Singh, D.K., 2009. Molluscicidal activity of Nutmeg and Mace (Myristica fragrans Houtt.) against the

vector snail Lymnaea acuminata. Journal of Herbs, spices and medicinal plants, 15, 177-186.

Kalesaraj, R., 1974. Screening of some indigenous plants for anthelmintic action against human Ascaris

lumbricoides. Indian J. Physiol. Pharmacol, 18: 129-31.

Kalesaraj, R., 1975. Screening of some indigenous plants for anthelmintic action against human Ascaris

lumbricoides. Part II Indian J. Physiol. Pharmacol, 19, 47-9.

Kirtikar, K.R., Basu, B.D., 1981. Indian medicinal Plants. Part II, Indian Press.

Kumar, P., Singh, D.K., 2006. Molluscicidal activity of Ferula asafoetida, Syzygium aromaticum and Carum carvi and

their active components against the snail Lymnaea acuminata. Chemosphere, 63, 1568-1574.

Kumar, P., Singh, V.K., Singh, D.K., 2009. Kinetics of enzyme inhibition by active molluscicidal agent ferulic acid,

umbelliferone, eugenol and limonene in the nervous tissues of snail Lymnaea acuminata. Phytotherapy

Research, 23 (2), 172 -177.

Kumar, P., Singh. V.K., Singh, D.K., 2011. Bait formulations of molluscicides and their effects on biochemical

changes in the ovotestis of snail Lymnaea acuminata (Mollusca; Gastropoda:Lymneidae). Rev. Inst. Med.

Trop. Sao. Paulo, 53 (5), 271-275.

Mas-Coma, S., Bargues, M.D., Valero, M. A., 2005. Fascioliasis and other plant borne trematode Zoonoses.

International Journal of Parasitology, 35, 1255-1278.

Mas-Coma, S., Valero, M.A., Bargues, M. D., 2009. Fasciola, Lymnaeids and human fascioliasis with a global

overview on disease transmission, Epidemiology, evolutionary genetics. Molecular Epidemiology and control,

In David Rollinson and Simon Iain Hay, Editors. Advances in Parasitology, Burlington: Academic Press, 69, 41-

Nadkarni, K.M., 1976. Indian Materia Medica. Vol I and II Popular Prakashan. Private Limited Bombay, India

Robertson, J.L., Russell, R.M., Preciter, H.K., Savin, N.E., 2007. Bioassay with arthropods data, 2nd Eds Taylar and

Francis, CRC Press, 1-224.

Saleem, M., Alam, A., Sultana, S., 2001. Asafoetida inhibits early events of carcinogenesis-a Chemopreventive

study. Journal of Life Science, 68, 1913-1921.

Singh, K., Singh, A., Singh, D.K., 1995. Molluscicides activity of different combination of the plant products used in

the molluscicides Pestoban. Biological Agriculture Horticulture, 12, 253-261.

Singh, O., Agarwal, R. A., 1981. Toxicity of certain pesticides to two economic species of snail in northern India.

Journal of Economic Entomology, 74, 568-571.

Singh, S., Singh, V.K., Singh, D.K., 1997. Molluscicidal activity of some common spice plants. Biological Agriculture

Horticulture, 14, 237- 249.

Singh, V.K., Singh, D.K., 1995. Characterization of allicin as a molluscicidal agent in Allium sativum (garlic). Biological

Agriculture Horticulture, 12, 119-131.

Sokal, R.R., Rohlf, F.J., 1996. Introduction of biostatistics, W.H. Freeman, San Francisco, Co, USA.

Srivastava, A.K., Singh, D.K., Singh, V.K., 2013. Abiotic factors and anti-reproductive action of bait containing

eugenol against Lymnaea acuminata. Scientific Journal of Biology Science, 2(4), 76-85.

Steenis-Kruseman, M.J.V., 1953. Select Indonesian medicinal plants organize. Science Research Indonesia Bulletin,

: 31.

Sunita, K., Singh, D.K., 2011. Fascioliasis control: In vivo and in vitro phytotherapy of vector snail to kill Fasciola

larva. Journal of parasitology research, 2011, 1-7.

Published

2013-05-29

How to Cite

Sunita, K. ., Kumar, P. ., & K. Singh, D. (2013). In vivo phytotherapy of snail by plant derived active components in control of fascioliasis. Scientific Journal of Veterinary Advances, 2(5), 61-67. Retrieved from https://www.sjournals.com/index.php/sjva/article/view/1467

Issue

Section

Original Article