Influence of Cymbopogon proximus extract on lipid profile, biochemical hematological and coagulation parameters of hyperlipidemic albino rats

Authors

  • Hager Tarek H. Ismail Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Alzeraa Street, Zagazig City, Sharkia Province, Postal Code 44511, Egypt

Keywords:

Cholestyramine, Cymbopogon proximus, Hypercholesterolemia, Hypolipidemic

Abstract

The objective of this work was to investigate the possible hypolipidemic activity and side effects of the C. proximus extract  alone and combined with cholestyramine with half therapeutic doses by studying changes in different biochemical, hematological and coagulation parameters beside histopathological evaluation. A total of 50 female albino rats divided into five equal groups: Gp.(1) control, Gp.(2) hyperlipidemic, Gp.(3) hyperlipidemia plus cholestyramine, Gp.(4) hyperlipidemia plus C.proximus extract, Gp.(5) hyperlipidemia plus half therapeutic doses of  cholestyramine and C. proximus extract. At the 29th day of the experiment, blood samples were collected and divided into three portions for biochemical, hematological and coagulation studies. Liver was dissected out for histopathological studies. Treatment of hyperlipidemic rats with C. proximus extract alone or combined with cholestyramine showed marked decrease in different lipid profile values with hepatoprotective activity, lowering lipid peroxidation, increasing antioxidant activity in addition to non significant change in erythrogram, platelets count, leukogram and coagulation markers in compare with hyperlipidemic group. It could be concluded that, combining treatment of C.proximus extract with cholestyramine as a synthetic drug with half therapeutic doses gave a complementary hypolipidemic effect with a fewer side effects than treatment with each therapeutic agent alone.

References

Abou-Shoer, M.I., Fathy, H.M., Omar, A.A., 2011. Extract-template modeling and pattern recognition in the assessment of (Cymbopogon proximus). Am. J. Anal. Chem., 2, 500-510.

Bancroft, J.D., Stevens, A., Turner, D.R., 1996. Theory and Practice of Histological Technique. 4th ed. Churchill Livingstone, New York, USA.

Bauer, J.D., 1982. Clinical laboratory methods. 9th ed. C.V. Mosby Co., Missouri, 555.

Cleuren, A.C.A., Blankevoort, V.T., van Diepen, J.A., 2015. Changes in dietary fat content rapidly alter the mouse plasma coagulation profile without affecting relative transcript levels of coagulation factors. Plose One. 10(7), 1-15.

Dhande, S.R., Gursahani, M.S., Kadam, V.J., 2014. Standardization of cholesterol suspension induced hyperlipidemia in rats. World J. Pharm. Pharmaceut. Sci., 3(12), 1107-1123.

Dhingra, D., Lamba, D., Kumar, R., Nath, P., Gauttam, S., 2014. Antihyperlipidemic activity of Aloe succotrina in rats: Possibly mediated by inhibition of HMG-CoA reductase. I. S. R. N. Pharmacol., 1-9.

Friedewald, W.T., Levy, R.I., Fredrickson, D.S., 1972. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin. Chem., 18(6), 499–502.

Ibrahim, F.Y., El-Khateeb, A.Y., 2013. Effect of herbal beverages of Foeniculum vulgare and Cymbopogon proximus on inhibition of calcium oxalate renal crystals formation in rats. Ann. Agric. Sci., 58(2), 221–229.

Kim, E., Tolhurst, A.T., Qin, L.Y., Chen, X., Febbraio, M., Cho1, S., 2008. CD36/Fatty acid translocase, an inflammatory mediator, is involved in hyperlipidemia-induced exacerbation in ischemic brain injury. J. Neurosci., 28(18), 4661– 4670.

Kothari, S., Jain, A.K., Mehta, S.C., Tonpay, S.D., 2011. Hypolipidemic effect of fresh Triticum aestivum (wheat) grass juice in hypercholesterolemic rats. Acta. Pol. Pharm., 68(2), 291-294.

Lin, Y., Mousa, S.S., Elshourbagy, N., Mousa, S.A., 2010. Current status and future directions in lipid management: Emphasizing low-density lipoproteins, high-density lipoproteins and triglycerides as targets for therapy. Vasc. Health. Risk. Manag., 6(1), 73–85.

Mansour, H.A., Newairy, A.A., Yousef, M.I., Sheweita, S.A., 2002. Biochemical study on the effects of some Egyptian herbs in alloxan-induced diabetic rats. Toxicol., 170(3), 221–228.

McKenney, J.M., 2007. Report of the national lipid association's safety task force: the nonstatins. Am. J. Cardiol., 99 (6 A), 1C-58C.

Newairy, A.A., Mansour, H.A., Yousef, M.I., Sheweita, S.A., 2002. Alterations of lipid profile in plasma and liver of diabetic rats: Effect of hypoglycemic herbs. J. Environ. Sci. Health. B., 37(5), 475-484.

Olorunnisola, O.S., Bradley, G., Afolayan, A.J., 2012. Protective effect of T. violacea rhizome extract against hypercholesterolemia-induced oxidative stress in wistar rats. Molecules., 17(5), 6033-6045.

Rafieian-Kopaei, M., Shahinfard, N., Rouhi-Boroujeni, H., Gharipour, M., Darvishzadeh-Boroujeni, P., 2014. Effects of Ferulago angulata extract on serum lipids and lipid peroxidation. Evid. Base. Compl. Alternat. Med., 1-4.

Ramakrishnan, S., Prasannan, K.G., Rajan, R., 2001. Textbook of Medical Biochemistry.3rd ed. Orient Longman, India.

Rang, H.P., Dale, M.M., Ritter, J.M., Flower, R.J., 2007. Rang and Dale’s Pharmacology. 6th ed. Churchill Livingstone, Elsevier, Edinburgh, 321.

Rezq, A.A., 2012. Beneficial health effects of fennel seeds (shamar) on male rats feeding high fat-diet. Med. J. Cairo Univ., 80(2), 101-111.

Rouhi-Boroujeni, H., Rouhi-Boroujeni, H., Heidarian, E., Mohammadizadeh, F., Rafieian-Kopaei, M., 2015. Herbs with anti-lipid effects and their interactions with statins as a chemical anti- hyperlipidemia group drugs: A systematic review. A.R.Y.A. Atheroscler. 11(4), 244-251.

Senecha, C., Shama, P.K., D’Souza, U.P., Shastry, C.S., 2012. Anticholesteremic and antilipidemic activity of stem bark extracts of Moringa oleifera in diet induced hyperlipidemia model in rats. I. J. P. C. S., 1(3), 567-574.

Shanmugasundaran, R., Kalpana Devi, V., Tresina Spris, P., Maruthupandian, A., Mohan, V.R., 2011. Antidiabetic, antihyperlipidemic and antioxidant activity of Senna auriculat (L.) roxb leaves in alloxan induced diabetic rats. Int. J. Pharm. Tech. Res., 3(2), 747 - 756.

Shepherd, 1989. Mechanism of action of bile acid sequestrans and other lipid-lowering drugs. Cardiol., 76(1), 65-74.

Sheweita, S.A., Newairy, A.A., Mansour, H.A., Yousef, M.I., 2002. Effect of some hypoglycemic herbs on the activity of phase I and II drug-metabolizing enzymes in alloxan-induced diabetic rats. Toxicol., 174(2), 131–139.

Shuang, L.I.U., Wei, H.O.U., Hua, Q.I.N., Ying, W.A.N.G., 2015. Oxidized LDL stimulates lipid peroxidation-derived DNA and protein adducts in human vascular endothelial and smooth muscle cells. J. Huazhong Univ. Sci. Technol., 35(2), 200-205.

Silveira, A., Karpe, F., Blombäck, M., Steiner, G., Walldius, G., Hamsten, A., 1994. Activation of coagulation factor VII during alimentary lipemia. J. Am. Heart. Assoc., 14(1), 60-69.

Singhal, R., Harrill, A.H., Menguy-Vacheron, F., Jayyosi, Z., Benzerdjeb, H., Watkins, P.B., 2014. Benign elevations in serum aminotransferases and biomarkers of hepatotoxicity in healthy volunteers treated with cholestyramine. B.M.C. Pharmacol. Toxicol., 15(42), 1-7.

Van Berge Henegouwen, G.P., Keppler, D., Leuschner, U., Paumgartner, G., Stiehl, A., 2011. Biology of Bile Acids in Health and Disease. Falk Symposium. Kluwer Publishers, Netherlands.

Webber, M., Krishnan, A., Thomas, N.G., Cheung, B.M.Y., 2010. Association between serum alkaline phosphatase and C-reactive protein in the United States national health and nutrition examination survey 2005–2006. Clin. Chem. Lab. Med., 48(2), 167–173.

Zahorec, R., 2001. Ratio of neutrophil to lymphocyte counts rapid and simple parameter of systemic inflammation and stress in critically ill. Bratisl. Lek. Listy., 102(1), 5-14.

Published

2016-09-08

How to Cite

Tarek H. Ismail, H. . (2016). Influence of Cymbopogon proximus extract on lipid profile, biochemical hematological and coagulation parameters of hyperlipidemic albino rats. Scientific Journal of Veterinary Advances, 5(9), 123-133. Retrieved from https://www.sjournals.com/index.php/sjva/article/view/245

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Original Article