Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin

Authors

  • Juliana de Moura Alonso UNESP, Univ. Estadual Paulista, School of Veterinary Medicine and Animal Science. Botucatu, São Paulo, Brazil
  • Regina Kiomi Takahira UNESP, Univ. Estadual Paulista, School of Veterinary Medicine and Animal Science. Botucatu, São Paulo, Brazil
  • Tatiane F. Bachiega UNESP, Univ. Estadual Paulista, Biosciences Institute. Botucatu, São Paulo, Brazil
  • Karoline Alves Rodrigues UNESP, Univ. Estadual Paulista, School of Veterinary Medicine and Animal Science. Botucatu, São Paulo, Brazil
  • Ana Lúcia Miluzzi Yamada UNESP, Univ. Estadual Paulista, School of Veterinary Medicine and Animal Science. Botucatu, São Paulo, Brazil
  • Ana Liz Garcia Alves UNESP, Univ. Estadual Paulista, School of Veterinary Medicine and Animal Science. Botucatu, São Paulo, Brazil
  • Marcos Jun Watanabe UNESP, Univ. Estadual Paulista, School of Veterinary Medicine and Animal Science. Botucatu, São Paulo, Brazil
  • Celso Antônio Rodrigues UNESP, Univ. Estadual Paulista, School of Veterinary Medicine and Animal Science. Botucatu, São Paulo, Brazil
  • Carlos Alberto Hussni UNESP, Univ. Estadual Paulista, School of Veterinary Medicine and Animal Science. Botucatu, São Paulo, Brazil

Keywords:

tissue plasminogen activator, horse, D-dimer, fibrinolysis, heparin plasminogen activator inhibitor

Abstract

This study sought to assess peritoneal fibrinolytic activity post-enterotomy of the small colon in equines treated with heparin. 10 adult equines were divided into 2 groups of 5 animals each: the control group (CG) and treated group (TG). Both groups underwent laparotomy and enterotomy of the small colon. The TG received subcutaneous heparin (150 IU/kg, bwt q. 12 hours, 5 days). The animals were evaluated for the peritoneal concentrations of tissue plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1) and D-dimer at the following time-points: prior to enterotomy (M0); 12 hours after (M1); 1 day after (M2); 2 days after (M3); 4 days after (M4); 6 days after (M5); 10 days after (M6) and 14 days after enterotomy (M7).A significant difference in tPA level was observed between the groups when all time-points were combined, with a median value of 2.59 IU/mL for the CG and 2.03 IU/mL for the TG. Although no significant difference was observed when the groups were compared at different time-points, smaller tPA and D-dimer values were observed for the TG during heparin treatment.Conclusions: In addition to the finding that the TG showed a lower tPA concentration and reduced D-dimer formation, it was concluded that heparin treatment decreased the formation of fibrin clots and peritoneal fibrinolytic activity.Because elevated D-dimer levels are directly related to a poor prognosis and high mortality rate, this study reinforced the relevance of the use of heparin in hypercoagulable states and following abdominal surgery.

References

Andrade, G.P., Strickland, S., 1986. Interaction of heparin with plasminogen activators and plasminogen: effects on the activation of plasminogen. Biochemistry 25, 4033–4040.

Aysan, E., Bektas, H., Ersoz, F., Sari, S., Hug, G., 2010. Effects of contractubex on the prevention of postoperative peritoneal adhesion. J. Surg. Res. 164(2), 193–197.

Bahadir, I., Oncel, M., Kement, M., 2007. Intra-abdominal use of taurolidine or heparin as alternative products to an antiadhesive barrier (Seprafilm) in adhesion prevention: an experimental study in mice. Dis. Colon Rectum 50(12), 2209–2214.

Caldwell, F.J., Mueller, P.O.E., 2010. Fibrinolytic responses of the equine peritoneum to abdominal surgery, surgical trauma, and intraperitoneal sodium hyaluronate. J. Equine Vet. Sci. 30(6), 298–304.

Cesarini, C., Monreal, L., Armengou, L., Delgado, M.A., Rios, J., Jose-Cunilleras, E., 2010. Association of admission plasma D-dimer concentration with diagnosis and outcome in horses with colic. J. Vet. Intern. Med. 24, 1490–1497.

Claunch, K.M., Mueller, P.O.E., 2012. Treating intra-abdominal adhesions: The surgeon’s dilemma. Equine Vet. Educ., 24(11), 552–555.

Delgado, M.A., Monreal, L., Armengou, L., Rios, J., Segura, D., 2009. Peritoneal D-dimer concentration for assessing peritoneal fibrinolytic activity in horses with colic. J. Vet. Int. Med. 23, 882–889.

Eggleston, R.B., Mueller, P.O., 2003. Prevention and treatment of gastrointestinal adhesions. Vet. Clin. North Am. Equine Pract. 19, 741–763.

Holmdahl, L., 1997. The role of fibrinolysis in adhesion formation. Eur. J. Surg. Suppl. 577, 24–31.

Holmdahl, L., Risberg, B., Beck, D.E., Burns, J.W., Chegini, N.,

DiZerega, G.S., Ellis, H., 1997. Adhesions: pathogenesis and prevention-panel discussion and summary. Eur. J. Surg. Suppl. 577, 56–62.

Ivarsson, M.L., Falk, P., Holmdahl, L., 2001. Response of the visceral peritoneum to abdominal surgery. Br. J. Surg. 88, 148–151.

Jansen, R.P., 1998. Failure of peritoneal irrigation with heparin during pelvic operations in young women to reduce adhesions. Surg. Gynecol. Obstet. 166, 154.

Kaptanoglu, L., Kucuk, H.F., Yegenoglu, A., Uzun, H., Eser, M., Mentes, C.V., Kurt, N., 2008. Effects of seprafilm and heparin in combination on intra-abdominal adhesions. Eur. Surg. Res. 41(2), 203–207.

Kement, M., Censur, Z., Oncel, M., Buyukokuroglu, M.E., Gezes, F.C., 2011. Heparin for adhesion prevention: comparison of three different dosages with Seprafilm in a murine model. Int. J. Surg. 9(3), 225–228.

Kutlay, J., Ozer, Y., Isik, B., Kargici, H., 2004. Comparative effectiveness of several agents for preventing postoperative adhesions. World J. Surg. 28, 662.

Majerus, P.W., 2006. Blood coagulation and anticoagulation, thrombolytic, and antiplatelet drugs, in: Brunton, L.L., Chabner, B.A., Bjorn, C. (Eds.), Goodman & Gilman's The Pharmacological Basis of Therapeutics, eleventh ed. McGraw Hill, New York.

Mitchell, R., Kumar, V., Fausto, N., Abbas, A.K., Aster, J., 2005. Inflamação aguda e crônica [Acute and chronic inflammation], in: Robbins & Cotran Fundamentos de Patologia: Bases Patológicas das Doenças [Robbins & Cotran Fundamentals of Pathology: Pathological Basis of Diseases], seventh ed. Elsevier, Rio de Janeiro, pp. 29–54.

Moore, B.R., Hinchcliff, K.W., 1994. Heparin: a review of its pharmacology and therapeutic use in horses. J. Vet. Intern. Med. 8(1), 26–35.

Opitiz, I., Van der Veen, H.C., Braumann, C., Ablassmaier, B., Fuhrer, K., Jacobi, C.A., 2003. The influence of adhesion prophylactic substances and taurolidine/heparin on local recurrence and intraperitoneal tumor growth after laparoscopic-assisted bowel resection of colon carcinoma in a rat model. Surg. End. Interv. Techn. 17(7), 1098–1104.

Pagano, M., Gauvreau, K., 2000. Principles of Biostatistics. Duxbury/Thomson Learning, California, pp. 285–301.

Parker, J., Fubini, S., Car, B.D., Erb, H.N., 1987. Prevention of intra-abdominal adhesions in ponies by low dose heparin therapy. Vet. Surg. 16, 459–462.

SAS Institute Inc., 2009. SAS/STAT® 9.2 User’s Guide, second ed. SAS Institute Inc., Cary, NC.

Shimomura, M., Takao, H.; Satoshi, I., Tomohiro, A., Kawaguchi, Y., Tokunaga, M., Sassada, T., Egi, H., Tanabe, K., Okajima, M., Ohdan, H., 2012. Preservation of peritoneal fibrinolysis owing to decreased transcription of plasminogen activator inhibitor-1 in peritoneal mesothelial cells suppresses postoperative adhesion formation in laparoscopic surgery. Surgery 153(3): 344–356.

Stokol, T., Erb, H.N., Wilde, L., Tornquist, S.J., Brooks, M., 2005. Evaluation of latex agglutination kits for the detection of fibrin(ogen) degradation products and D-dimer in healthy horses and horses with severe colic. Vet. Clin. Pathol. 34, 375–382.

Ward, B.C., Panitch, A., 2011. Abdominal adhesions: current and novel therapies. J. Surg. Res. 165, 91–111.

Watson, A., Vandekerckhove, P., Lilford, R., 2000. Liquid and fluid agents for preventing adhesions after surgery for subfertility. Cochrane Database Syst. Rev. (3), CD001298.

Young, D.R., Richardson, D.W., Markel, M.D., 1989. The effect of low-dose heparin therapy on complication and survival rates in horses following exploratory celiotomy. Equine Vet. J. Suppl. 7, 91–93.

Published

2014-01-29

How to Cite

Moura Alonso, J. de ., Kiomi Takahira, R. ., F. Bachiega, T. ., Alves Rodrigues, K. ., Miluzzi Yamada, A. L. ., Garcia Alves, A. L. ., Jun Watanabe, M. ., Antônio Rodrigues, C. ., & Alberto Hussni, C. . (2014). Peritoneal fibrinolytic activity in equines subjected to small colon enterotomy and treated with heparin. Scientific Journal of Veterinary Advances, 3(1), 6-13. Retrieved from http://www.sjournals.com/index.php/sjva/article/view/1449

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Original Article