In vivo phytotherapy of snail by plant derived active components in control of fascioliasis

Abstract

Fascioliasis is worldwide zoonotic disease, which caused great economic loss to human being. In vivo killing of Fasciola larva (redia and cercaria) is one of the effective methods to control of fascioliasis. In vivo toxicity of different binary combinations (1:1ratio) of plant derived active components such as citral, ferulic acid, umbelliferone; azadirachtin and allicin were tested against Fasciola larva  (redia and cercaria) in snail Lymnaea acuminata. Mortality of larvae were observed at 2h, 4h, 6h and 8h of treatment. In vivo exposure of azadirachtin + allicin (1:1 ratio) was highly toxic against redia and cercaria (8h LC₅₀ 0.007and 0.006 mg/L). Combinations of citral + umbelliferone, cirtal + ferulic acid were less toxicity against redia and cercaria larva than azadirachtin + allicin. In vivo use of these larvicidal active components in the binary combinations at sublethal does will be more helpful in controlling the redia/cercaria than their individual components.