Antimicrobial activity bioactive compounds produced by Exiguobacterium acetylicum PTCC1756 against pathogenic bacteria

Authors

  • M. Azizollahi Aliabadi Department of Microbiology, Faculty of Basic Science Lahijan Branch, Islamic Azad University, Lahijan, IRAN
  • M. Faezi Ghasemi Department of Microbiology, Faculty of Basic Science Lahijan Branch, Islamic Azad University, Lahijan, IRAN
  • KH. Isaazadeh Department of Microbiology, Faculty of Basic Science Lahijan Branch, Islamic Azad University, Lahijan, IRAN

Keywords:

E. acetylicum, antibacterial, Disk Diffusion Agar and Well Diffusion Agar

Abstract

Almost all forms of life in the marine environment (bacteria, algae, fungi, etc…) have been investigated for their natural product content. In the last several decades, plants, animals and microbes from the marine environment have revealed a portion of what is clearly a tremendous source of structurally diverse and bioactive secondary metabolites. Exiguobacterium acetylicum is a Gram positive, rhizospheric, yellow pigmented bacteria isolated from an apple orchard rhizospheric soil, facultative aerobic, motile with peritrichous flagella and their growth ranges are from _2.5 to 40 . These micro organisms In separate invitro assays it was found to inhibit the growth and development of Rhizoctonia solaniSclerotium rolfsii, Pythium and Fusarium oxysporum. The volatile compound produced by the bacterium was found to be the most potent in inhibiting the hyphal fungus and pathogens bacteria. Present study aims to isolated and detect the surfactants produced by Exiguobacterium acetylicum PTCC 1756 For this purpose, a suspension equal to 0.5 McFarland was prepared from the fresh growth of following standard clinic strains, Escherichia coli PTCC 1533, Shigella Flexneri PTCC 1234, Staphylococcus aureous PTCC 1112,  Salmonella enterica subsp. enterica serovar Paratyphi B PTCC 1231 and the plated on the Muller Hinton agar.  Supernatant of Eacetylicum growth was prepared in 15000 ppm for 30 minutes and was utilized. To study on the antimicrobial activity of  Eacetylicum PTCC1756, Muller- Hinton agar was used. For this purpose, Disk Diffusion Agar and Well Diffusion Agar techniques were used to define MIC. These micro organisms growth supernatant displayed a good antimicrobial property and the maximum effect was observed during the well diffusion technique against the standard strain Salmonella enterica subsp enterica serovar paratyphi PTCC 1231 with 8.5 mm no growth halo.

References

Anderson, C.R., Cook, G.M., 2004. Isolation and characterization of arsenate-reducing bacteria from arsenic-contaminated sites in New Zealand. Curr. Microbiol., 48,341–347.

Chaturvedi, P., Shivaji, S., 2006. Exiguobacterium indicum sp nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan mountain ranges of India. Int. J. Syst. Evol. Microbiol., 56,2765–2770.

De Weger, L.A., vander, B.A., Dekkers, L.C., et al., 1995. Colonization of the rhizosphere of crop plants by plant-beneficial pseudomonads. FEMS Microbiol. Ecol., 17,221–228.

Farrow, J.A.E., Wallbanks, S., Collins, M.D., 1994. Phylogenetic interrelationships of round-spore-forming bacilli containing cell-walls based on lysine and the non-spore-forming genera Caryophanon, Exiguobacterium, Kurthia, and Planococcus. Int. J. Syst. Bacteriol., 44,74–82.

Gerhardson, B., 2002. Biological substitutes for pesticides. Trends Biotechnol., 20,338–343. doi,10.1016/S0167-7799(02)02021-8.

Glick, B., 1995. The enhancement of plant growth by free-living bacteria. Can J Microbiol., 41,109–117.

Javanbakht Yousefi, S., Ghasemi, F.M., Amir Mozaffari, N., 2009. Biological evaluation of bioactive substances produced in bacteria isolated from coastal waters of the Caspian. J. Biolog. Sci. clones, Winter 2009; since the third quarter numbers.

Kasana, R.C., Yadav, S.K., 2007. Isolation of a psychrotrophic Exiguobacterium sp. SKPB5 (MTCC 7803) and characterization of its alkaline protease. Curr. Microbiol., 54,224–229.

Kumar, A., Singh, V., Kumar, R., 2006. Characterization of an alkaliphile, Exiguobacterium sp. and it’s application in bioremediation. In. Int. confer. Extremoph., Brest, France, p L89.

Pandey, A., Trivedi, P., Kumar, B., et al., 2006. Soil microbial diversity from the Himalaya, need for documentation and conservation. NBA Bulletin no 5. National Biodiversity Authority Chennai., Tamilnadu, India, p 64.

Rodriguez, D.F., Goris, J., Vishnivetskaya., 2006. Characterization of Exiguobacterium isolates from the Siberian permafrost. Description of Exiguobacterium sibiricum sp. nov. Extremophiles., 2006; 10,285–294.

Selvakumar, G., Joshi, P., Nazim, S., Mishra, P.K., Kundu, S., Gupta, H.S., 2009. Exiguobacterium acetylicum strain 1P (MTCC 8707) a novel bacterial antagonist from the North Western Indian Himalayas. World J. Microbiol. Biotechnol., 2009, 25,131–137.

Vishnivetskaya, T.A., Kathariou, S., 2005. Putative transposases conserved in Exiguobacterium isolates from ancient Siberian permafrost and from contemporary surface habitats. Appl. Env. Microbiol., 71, 6954–6962.

Vishnivetskaya, T.A., Ramaley, R., Rodrigues, D.F., Tiedje, J.M., Kathariou, S., 2005. Exiguobacterium from frozen subsurface sediments (Siberian permafrost) and from other sources have growth temperature ranges reflective of the environmental thermocline of their origin. In, The joint international symposia for subsurface microbiology (ISSM 2005) and environmental biogeochemistry (ISEB XVII), Jackson Hole, WY, p 254.

Vishnivetskaya, T.A., Siletzky, R., Jefferies, N., Tiedje, J.M., Kathariou, S., 2007. Effect of low temperature and culture media on the growth and freeze-thawing tolerance of Exiguobacterium strains.Cryobiol.,54,234–240.

Wada, M., Yoshizumi, A., Furukawa, Y., Kawabata, H., Ueda, M., Takagi, H., Nakamori, S., 2004. Cloning and overexpression of the Exiguobacterium sp. F42 gene encoding a new short chain dehydrogenase, which catalyzes the stereoselective reduction of ethyl 3-oxo-3-(2-thienyl)propanoate to ethyl (S)-3-hydroxy-3-(2- thienyl)propanoate. Biosci Biotechnol Biochem., 68,1481–1488.

Yumoto, I., Hishinuma-Narisawa, M., Hirota, K., Shingyo, T., Takebe, F., Nodasaka, Y., Matsuyama, H., Hara, I., 2004. Exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity. Int. J. Syst. Evol. Microbiol., 2004, 54,2013–2017. doi,10.1099/ijs.0.63129-0.

Published

2014-04-25

How to Cite

Azizollahi Aliabadi, M. ., Faezi Ghasemi, M. ., & Isaazadeh, K. . (2014). Antimicrobial activity bioactive compounds produced by Exiguobacterium acetylicum PTCC1756 against pathogenic bacteria. Scientific Journal of Microbiology, 3(4), 55-61. Retrieved from https://www.sjournals.com/index.php/sjm/article/view/1369

Issue

Section

Original Article