Bacterial diversity on fishes and in waters from southern rivers in Mali

Authors

  • R. Fané Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technology of Bamako, BPE. 3206 Bamako-Mali
  • F. Samake Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technology of Bamako, BPE. 3206 Bamako-Mali
  • A. H. Babana Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technology of Bamako, BPE. 3206 Bamako-Mali
  • Y. Sanogo Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technology of Bamako, BPE. 3206 Bamako-Mali
  • D. Traoré Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technology of Bamako, BPE. 3206 Bamako-Mali
  • A. H. Dicko Laboratory of Research in Microbiology and Microbial Biotechnology, Faculty of Sciences and Techniques, University of Sciences, Techniques and Technology of Bamako, BPE. 3206 Bamako-Mali

Keywords:

Diversity, microbial flora, fish,rivers of the south, Mali

Abstract

Microbial diversity of fishes of river water from Bagoé, Baoulé and Sankarani in the Niger River basin in southern Mali, was investigated. Gills and intestines were sampled on forty-three (43) fishes. Also, five water samples were collected for microbial diversity analysis. Total mesophilic flora and fauna, indicator contamination were investigated in water and in the gills and intestines fish species of economic interest, freshly caught. Conventional microbiological analysis methods were used. Biochemical tests were performed for bacterial identification. The main results obtained were: (i) the bacterial density in fishes was 152.106 to 66.106 CFU/g in gills and 112±53.106 to 50±8.5.106 CFU / g in intestine, (ii ) bacterial flora in fishes from rivers is composed 61% of Gram-negative and 39% Gram-positive bacilli. River waters were composed of 91% of Gram-negative bacilli and 9% of Gram-positive bacilli. In waters and fishes investigated, the different bacteria identified belong to three bacterial families: Pseudomonadaceae, Enterobacteriaceae and Bacillaceae. Bacterial flora of fishes from southern rivers was found similar to those of investigated river waters. But, the bacterial flora of the river waters had a higher proportion of Gram-negative bacilli than those in fishes.

References

Bryant, R.D., McGroarty, K.M., Costeron, J.W., Laishley, E.J., 1983. Isolation and characterization of a new acidophilic Thiobacillus species (T. albertis). Can . J. Microbiol., 29, 1159-1170.

Durand, J., Toumanoff, C., 1967. Contribution à l’étude de la flore bactérienne des poisons du Niger Supérieur. Ch. O.R.S.T.O.M., sér. Hydrobiol., vol. I, n°14

FAO., 2006. La situation mondiale des pêches et de l'aquaculture 180p.

Hugh, R., Leifson, E., 1953. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J. Bacterial. 66, 24-26.

KING, E.O., Ward, M.K., Raney, D.E., 1954. Two simple media for demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med., 44, 301-7.

Organisation Mondiale de la Santé, Organisation des Nations Unie pour l’Alimentation et l’Agriculture., 2009. Code d’usage pour les poissons et les produits de la pêche, première édition, 170p.

Reynolds, C.S., Jaworsky, G.H.M., Cmiech, H.A., Leedale, G.F., 1981. On the annual cycle of the blue-green algae Microcystis aeruginosa Kütz. emend. Elenkin. Philos. Trans. R. Soc. London Ser., B, 293, 419–477.

Shewan, J.M, 1977. The Bacteriology of fresh and spoiling biochemical changes induced by bacterial action. In proceeding of conference on the handling processing and marketing of tropical fish. London, Trop. Prod. Inst., PP. 51-6.

Published

2013-10-29

How to Cite

Fané, R. ., Samake, F. ., H. Babana, A., Sanogo, Y. ., Traoré, D. ., & H. Dicko, A. (2013). Bacterial diversity on fishes and in waters from southern rivers in Mali. Scientific Journal of Microbiology, 2(10), 187-193. Retrieved from https://www.sjournals.com/index.php/sjm/article/view/1383

Issue

Section

Original Article