Evaluation of Erythromycin–resistant and Chloramphenicol-sensitivity on Agrobacterium vitis in Iran
Keywords:Agrobacterium vitis, Erythromycin, Chloramphenicol, Iran
The purpose of this study was to determine the prevalence of Erythromycin and Chloramphenicol resistant/sensitivity of Agrobacterium vitis that isolated from environmental samples in Iran vineyards. From May to June 2014, 20 samples isolated from Vineyards of five grape cultures from west of Iran. After identification of Agrobacterium vitis by biochemical, microbiological and molecular methods, antibiotic susceptibility testing was performed by Kirby-Bauer disk diffusion test for these antibiotics. The lowest and highest resistance was seen for Chloramphenicol (n=1) and Erythromycin(n=10). The results showed that the frequency of Erythromycin resistance is very high in Agrobacterium vitis strains isolated from vineyards that had crown Gall in west of Iran. Thus, urgent interventions are needed to keep the emergence and transmission of these isolates to a minimum. The lowest resistance on Chloramphenicol in Agrobacterium vitis strains showed that we can use it for urgent occasions.
Ataee, R., Derakhshanpoor, J.M., Tavana, A., Eidee, A., 2011. Antibacterial effects of Nanoparticles of calcium carbonate on Agrobacterium tumefacien. Iran. J. Milit. Med., 13(2), 65-70.
Bazzi, C., Piazza, C., Burr, T.J., 1987. Detection of Agrobacterium tumefaciensin grapevine cuttings. Bull. OEPP/EPPO; 17, 105- 112.
Burr, T.J., Katz, B.H., 1983. Isolation of Agrobacterium tumefaciensbiovar 3 from grapevine galls and sap, and from vineyard soil. Phytopathol., 73, 163-165.
Burr, T.J., Katz, B.H., 1984. Grapevine cuttings as potential sites of survival and means of dissemination of Agrobacterium tumefaciens. Plant. Dis., 68, 976-978.
Burr, T.J., Katz, B.H., Bishop, A.L., Meyers, C.A., Mittak, V.L., 1988. Effect of shoot age and tip culture propagation on grapes on systemic infestation by Agrobacterium tumefaciens biovar 3. Am. J. Enol. Vitic., 39, 67-70.
Burr, T.J., Reid, C.L., 1993. Biological control of grape crown gall with nontumorigenicAgrobacterium vitis strain F2/5. Am. J. Enol. Vitic., 45, 213-219.
Clinical and Laboratory Standards Institute, 2011. Performance standards for antimicrobial susceptibility testing, 21st informational supplement. CLSI document M100-S21. Clinical and Laboratory Standards Institute, Wayne, PA.
Fraschini, F., Scaglione, F., Ferrara, F., Marelli, O., Braga, P., Teodori, F., 1986. Evaluation of the immunostimulating activity of erythromycin in man. Chemother., 32, 286–290.
Moore, L.W., 1988. Use of Agrobacterium radiobacter inagricultural ecosystems. Microbiol. Sci., 5(3), 92-5.
Szegedi, S., Bottka, 2002. Detection of Agrobacterium vitis by polymerase chain reaction in grapevine bleeding sap after isolation on a semi selective medium. Visit; 41(1), 37-42.
How to Cite
Copyright (c) 2020 Fatemeh Ghafarneghad Mogadam, Reza Talebe, Amir Tokmachi
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.