In vitro evaluation of the acaricidal effect of vegetal oils extracted from the kernel of Thevetia peruviana and Annona muricata on the Rhipicephalus (Boophilus) microplus larvae

Authors

  • Kossi Justin Adinci University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Yao Akpo University of Parakou (UP), Faculty of Agronomy, Po Box 123 Parakou, Benin
  • Arétas Tonouhewa University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Roland Eric Yessinou University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Philippe Sessou University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Mahuto Yovo Laboratory of Research and Study in Applied Chemistry. Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin
  • Safiou Bienvenu Adehan National Institute for Scientific Research, Research Center of Agonkanmey (CRA / INRAB), Abomey-Calavi, Benin
  • Camus Adoligbe University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Guy Appolinaire Mensah National Institute for Scientific Research, Research Center of Agonkanmey (CRA / INRAB), Abomey-Calavi, Benin
  • Marc Napoléon Assogba University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Issaka Youssao Abdou Karim University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin
  • Souaïbou Farougou University of Abomey-Calavi (UAC), Polytechnic School of Abomey-Calavi (EPAC) Production and Animal Health Department, Applied Biology Research Laboratory (LARBA) 01 Po. Box: 2009 Cotonou, Benin

Keywords:

Rhipicephalus (Boophilus) microplus, Cattle, Vegetable oil, DL50, DL90, Acaricidal, Benin

Abstract

The objective of this work was to study the acaricide activity of oils extracted from kernels of T. peruviana and A. muricata with the hexane at different concentrations on Rhipicephalus (Boophilus) microplus larvae aged from 14 to 21 days. For this purpose, the Larva Immersion Test was performed. Twice dilutions of both oils were tested from a starting dilution of 40% to 2.5%. There were three repetitions for each concentration and for each oil. The control solution in which these larvae were treated consisted of Tween-20, diluted at 2% in distilled water. The experimental groups were stored in an incubator at 27 ± 1 °C and at 85-90% of relative humidity for 24 hours. The larvae mortality was greater than 55% in all tested groups and reached 100% at the 40% concentration for A. muricata, while the control group had a 0% mortality. The LC50 and CL90 obtained were respectively 4.0331% and 10.7594% for A. muricata and 7.0942% and 41.4247% respectively for T. peruviana. These results clearly indicate that these oils all have acaricidal effects on Rhipicephalus (Boophilus) microplus larvae. However, A. muricata oil is the most toxic. It can therefore be used as an effective alternative to control the Rhipicephalus (Boophilus) microplus tick and there is a high probability that it can be used for other ticks affecting cattle and even other ectoparasites in Benin and worldwide, thereby reducing the use of synthetic acaricides which are toxic to the environment and ineffective against this tick.

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Published

2017-03-22

How to Cite

Justin Adinci, K. ., Akpo, Y. ., Tonouhewa, A. ., Eric Yessinou, R. ., Sessou, P. ., Yovo, M. ., Bienvenu Adehan, S. ., Adoligbe, C. ., Appolinaire Mensah, G. ., Napoléon Assogba, M. ., Abdou Karim, I. Y. ., & Farougou, S. . (2017). In vitro evaluation of the acaricidal effect of vegetal oils extracted from the kernel of Thevetia peruviana and Annona muricata on the Rhipicephalus (Boophilus) microplus larvae. Scientific Journal of Veterinary Advances, 6(3), 162-169. Retrieved from http://www.sjournals.com/index.php/sjva/article/view/240

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Vol. 6 No. 3 (2017): March

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Original Article

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Copyright (c) 2020 Kossi Justin Adinci, Yao Akpo, Arétas Tonouhewa, Roland Eric Yessinou, Philippe Sessou, Mahuto Yovo, Safiou Bienvenu Adehan, Camus Adoligbe, Guy Appolinaire Mensah, Marc Napoléon Assogba, Issaka Youssao Abdou Karim, Souaïbou Farougou

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